Lab exercise - Coursework Example In this process, the colonies are placed on plates and then for each dilution the number of counting units are determined by counting the units on the plates. The count should be between 30 and 800. The counted values are then recorded in a table in excel spreadsheet. In cases where the numbers of the CFUs is greater than 1000, then the value is recorded as TNTC i.e. Too Numerous to Count. In cases where the count is less than 10, the value is recorded but it is not utilized in the final calculation. The results are recorded in a table just like the one shown below Sterile transfer technique used in the transformation of bacteria from one location to another. This ensures no additional bacteria contaminates the one (bacteria) in transit. Also, this technique is used to prevent the bacteria from escaping and infecting the environment. The success of the sterile technique is dependent on several factors. The factors that facilitates the success of sterile transfer experiments are as highlighted below; ii) All the ends of loops and all the transfer loops must be flamed. Also, caution should be taken such that the tubes are kept open only in the minimum amount of time possible. When the tubes are open, they should be inclined at 45
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